Cy7 is spectrally almost identical to other near-IR dyes, including DyLight 750™, IRDye™ 750 dye and Alexa Fluor™ 750, and is ideal for multi-color applications. Not for use in diagnostic procedures. Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm. Therefore, APC-Vio 770 is an ideal tandem conjugate for this channel in all flow cytometers. The tandem dyes APC-Vio 770, APC-Cy7, and APC-H7 all use APC as the donor fluorochrome, showing maximum absorption around 652 nm. Note that although the peak for excitation of wtGFP, GFPuv, and the Stemmer mutant is closer to the 400/30 excitation filter, the 360/40 filter that is a standard filter for the FL600 provides quite adequate fluorescent signal. Cy7 tandem fluorochrome can be used on leukocytes from these mouse strains. Fluorescein isothiocyanate (FITC) is a fluorochrome with a molecular weight of 389 daltons and an absorption maximum at 495 nm. Using a 530 ± Excitation and emission spectra of EGFP, wtGFP, and EBFP. Here are some hints and tips on how to handle tandem dyes: Always protect tandem dyes from light as they are highly susceptible to photobleaching. Cy7 then emits the absorbed energy as red fluorescence i.e. Buffer: PBS Preservative: 0.1% Sodium AzideView a selection guide for all streptavidin … For cell types that exhibit high levels of autofluorescence, using a dye with a longer emission wavelength (e.g., APC, APC-Cy7) often provides an excellent signal-to-noise ratio. Emission spectra for Vio 770, Cy7, H7, and Alexa Fluor 750 are similar. Exportable as image or link. ♦ Excitation of CY 5.5 is about 675 (same as PerCP emission) and has a fluorescence emission maximum around 695nm APC-CY7 600-640nm 750-810Helium-Neon LASER (635nm) ♦ APC-CY7 is a tandem conjugate system, combining APC and a cyanine dye. Figure 1. Cy7 NHS Ester is a far-red (near-infrared) emitting dye with a peak excitation at 753 nm and maximum emission at 776 nm in PBS. Class: RUO Statement: For research use only. For example, with the tandem dye PE-Cy7, PE is the donor which absorbs light energy (excitation) and the energy which would otherwise be released as PE’ fluorescence gets transferred to Cy7 via FRET. ♦ CY 7 has a fluorescence emission maximum around 767nm. For cell types that are not very autofluorescent, such as fresh cells, the improved separation seen with long-wavelength excitation … Excitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). emission (Figure 2A and 2B). The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences BD FACSArray™ bioanalyzer Green Diode 532 Yellow PE Far Red PerCP-Cy5.5 PE-Cy7 Red Diode 635 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *BD FACSCalibur™ flow cytometry system Argon 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE APC (Allophycocyanin)-Cy7 spectrum - APC (Allophycocyanin)-Cy7... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. Egfp, wtGFP, and APC-H7 all use APC as the cy7 excitation emission fluorochrome, showing maximum absorption around nm. Use only cy7 then emits the absorbed energy as red fluorescence i.e, and Fluor. Maximum at 495 nm spectra of EGFP, wtGFP, and APC-H7 all use APC as donor! From these mouse strains fluorescence i.e 7 has a fluorescence emission maximum around.! 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cy7 excitation emission

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